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  • Title: Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.
    Author: Ronson CW, Nixon BT, Albright LM, Ausubel FM.
    Journal: J Bacteriol; 1987 Jun; 169(6):2424-31. PubMed ID: 3034856.
    Abstract:
    We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.
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