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  • Title: Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter.
    Author: Ishikawa T, Hama H, Tsuda M, Tsuchiya T.
    Journal: J Biol Chem; 1987 Jun 05; 262(16):7443-6. PubMed ID: 3034895.
    Abstract:
    A mutant of Escherichia coli with defective Na+/H+ antiporter was isolated. The rationale for its isolation was that cells possessing defective Na+/H+ antiporter, which is essential for establishment of a Na+ gradient, could not grow with a carbon source that was taken up with Na+. The mutant had no appreciable Na+/H+ antiporter activity, but its K+/H+ antiporter and Ca2+/H+ antiporter activities were normal. Judging from the reversion frequency, the defect seems to be due to a single mutation. The mutant could not grow at alkaline pH. Therefore, the Na+/H+ antiporter, but not the K+/H+ antiporter or the Ca2+/H+ antiporter, seems to be responsible for pH regulation in alkaline medium. This mutant will be useful for cloning the Na+/H+ antiporter gene and for detection of Na+-substrate cotransport systems.
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