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  • Title: CRISPR/Cas9-Based Gene Dropout Screens.
    Author: Wu K, Malek SN.
    Journal: Methods Mol Biol; 2019; 1881():185-200. PubMed ID: 30350207.
    Abstract:
    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-based technology enables efficient and precise perturbations of target genomic sites. Combining the endonuclease Cas9 and a pooled guide RNA library allows for systematic screenings of genes associated with a growth disadvantage or lethal phenotype under various conditions in organisms and tissues. Here, we describe a complete protocol for scalable CRISPR/Cas9-based dropout screening for essential genes from focused genomic regions to whole genomes.
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