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  • Title: Muconic Acid Production Using Gene-Level Fusion Proteins in Escherichia coli.
    Author: Fujiwara R, Noda S, Tanaka T, Kondo A.
    Journal: ACS Synth Biol; 2018 Nov 16; 7(11):2698-2705. PubMed ID: 30350569.
    Abstract:
    This study reports on the improving of muconic acid (MA) production by using metabolically engineered Escherichia coli. Three MA synthesis pathways separately were introduced into E. coli. After 72 h of cultivation, two of the three strains, i.e., one carrying the Pathway 1 (1.00 g/L) and the other carrying the Pathway 3 (1.34 g/L) produced MA. To increase MA production, the enzymes of the shikimate pathway (AroC and AroD) were overexpressed in these strains. Although the overexpression of AroC increased the MA production (1.59 g/L) by the Pathway 1, AroD overexpression decreased it by the Pathway 3. The metabolic channeling using gene-level fusion proteins additionally increased the MA production. In the pathway 1 and pH-controlled cultures, the overexpression of a fusion protein (AroC and MenF) increased the MA production from 20 g/L glucose to >3 and 4.45 g/L, respectively. These results suggest that the metabolic channeling approach is a promising strategy to increase the yield of the target compound.
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