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  • Title: Development of SPE method for the extraction of phosphorothioate oligonucleotides from serum samples.
    Author: Nuckowski Ł, Kaczmarkiewicz A, Studzińska S.
    Journal: Bioanalysis; 2018 Oct 01; 10(20):1667-1677. PubMed ID: 30354278.
    Abstract:
    AIM: Comprehensive development of a method for SPE extraction of antisense phosphorothioate oligonucleotide and its metabolites and their determination with the use of UHPLC. RESULTS: Polymer-based adsorbent and high percentage of methanol in elution solvent provided high recoveries compared with silica-based octadecyl cartridge. As to the type and concentration of ion pair reagent and organic solvent, the mixture of 5 mM of N,N-dimethylbutylamine/150 mM of 1,1,1,3,3,3-hexafluoroisopropanol and methanol was selected. Relatively high recoveries in the range of 79.2-81.2% with the SDs of 3.4-6.2% were obtained for the oligonucleotide and its metabolites extracted from human serum. CONCLUSION: The developed method may be successfully applied for routine analysis of antisense oligonucleotides in serum since it is relatively easy, quick and reliable.
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