These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Expressing plasmid vectors on the basis of Escherichia coli beta-galactosidase gene fragments].
    Author: Chakhmakhcheva OG, Mirskikh OV, Chiong NH, Efimov VA.
    Journal: Bioorg Khim; 1987 Mar; 13(3):350-8. PubMed ID: 3036168.
    Abstract:
    With the use of synthetic DNA fragments, a set of new plasmid vectors has been obtained. The vectors provided high level expression of peptides and small proteins in E. coli as fusions with fragments of beta-galactosidase of various length. These vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. The yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total amount of cellular protein.
    [Abstract] [Full Text] [Related] [New Search]