These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Estrogen sulfates: biological and ultrastructural responses and metabolism in MCF-7 human breast cancer cells.
    Author: Pasqualini JR, Gelly C, Lecerf F.
    Journal: Breast Cancer Res Treat; 1986; 8(3):233-40. PubMed ID: 3036286.
    Abstract:
    The biological effects and ultrastructural alterations by different estrogen-3-sulfates (E1-3-S and E2-3-S) and estradiol-17-sulfate (E2-17-S) were studied in the MCF-7 mammary cancer cell line in culture. The estrogen-3-sulfates very significantly stimulated the progesterone receptor (PR). The values (in pmoles/mg DNA +/- SE) were: control, 0.46 +/- 0.09; E1-3-S, 2.24 +/- 0.30, and E2-3-S, 2.56 +/- 0.45. The value of PR after E2-17-S incubation (0.56 +/- 0.24) was similar to the non-treated cells. The PR values obtained by the incubation of unconjugated estrone and estradiol were: 2.63 +/- 0.45 and 2.27 +/- 0.36, respectively. Analysis of the unconjugated estrogens in the medium indicated significant hydrolysis of estrogen-3-sulfates but not of E2-17-S. Using [3H]-E1-3-S, an important transformation was observed inside the cells, a great part being converted to estradiol (greater than 60% in the nuclear fraction). Electron microscopic examination indicated alterations in the secretory system after incubation with estrogen-3-sulfates similar to those obtained with unconjugated estradiol. The effect provoked by E2-17-S was significantly less than for the other sulfates. As estrogen sulfates are quantitatively the most important form of estrogens in the mammary gland, it is suggested that estrogen-3-sulfates play an important role in the biological responses to estrogens in breast cancer.
    [Abstract] [Full Text] [Related] [New Search]