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  • Title: A rapid and precise assay for calmodulin and cyclic AMP phosphodiesterase on a centrifugal analyser.
    Author: Walker SW, Milner SW, Howie AF.
    Journal: Clin Chim Acta; 1987 May 15; 164(3):305-13. PubMed ID: 3036405.
    Abstract:
    An assay for cyclic AMP phosphodiesterase in biological samples is described. The method is a continuous, spectrophotometric assay adapted for use on a centrifugal analyser. Using the calmodulin-activated cyclic AMP phosphodiesterase prepared from pig brain by DEAE-cellulose chromatography, and a standard calmodulin preparation from the same source, a rapid assay for calmodulin is described. The calmodulin assay has a working range of 2 to 6 mg/l calmodulin in the sample. The within-batch CV is less than 5% and the between-batch CV is 7.0% or less.
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