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  • Title: A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles.
    Author: Qu F, Xia W, Xia L, You J, Han W.
    Journal: Talanta; 2019 Feb 01; 193():37-43. PubMed ID: 30368295.
    Abstract:
    Heparin (Hep) is a widely applied anticoagulant and the quantification of heparin concentration is pivotal for clinical use. In this work, silicon nanoparticles (SiNPs) modified by the amino groups and glutathione-capped gold nanoclusters (GSH-AuNCs) are able to self-assemble into spherical particle structures via the electrostatic interaction, resulting in the aggregation-enhanced emission (AEE) of GSH-AuNCs. However, Hep, a highly sulfated glycosaminoglycan with much more negative charges, can bind with the SiNPs and inhibit the aggregation. As a result, it causes the AEE quenching of GSH-AuNCs at 570 nm but the SiNPs keep their own blue fluorescence at 450 nm. Thus, the SiNPs can act as an internal reference and the GSH-AuNCs are used as a signal probe in this process. The ratiometric fluorescent signal (I570/I450) change of the nanohybrid probe is positively correlated with Hep concentrations in the range from 6.44 ng/mL to 96.6 ng/mL with the detection limit of 3.29 ng/mL. As expected, this strategy shows good sensitivity and selectivity, and it is also successfully applied to detect Hep in Hep sodium injection and human serum samples with good recoveries.
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