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  • Title: Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
    Author: Saga Y, Tung JS, Shen FW, Boyse EA.
    Journal: Proc Natl Acad Sci U S A; 1987 Aug; 84(15):5364-8. PubMed ID: 3037546.
    Abstract:
    Previous inferences that Ly-5 glycoprotein isoforms of murine hematopoietic cells are generated by alternative splicing of primary transcripts of a single Ly-5 gene are supported by the present study. A cDNA library was prepared from B cells by extension from primer representing a known T-cell cDNA sequence. Three different Ly-5 clones from this library included sequences missing in T-cell cDNA clones. From the constitution of cDNA clones and of the Ly-5 gene, and from S1 nuclease mapping, it is concluded that at least two exons, provisionally numbered Ex-6(B) and Ex-7(B), in the 5'-proximal region are mainly represented in mRNA of the B-cell lines examined but not of the T-cell lines examined. Also, exons 1 and 2 appear to be used alternatively in different species of B-cell mRNA and probably also in different species of T-cell mRNA.
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