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  • Title: [Genotyping Clostridium perfringens strains isolated from patients with symptoms of food poisoning and food samples].
    Author: Brodzik K, Augustynowicz E.
    Journal: Med Dosw Mikrobiol; 2016; 68(3-4):191-201. PubMed ID: 30376619.
    Abstract:
    INTRODUCTION: Clostridium perfringens strains that produce enterotoxin often cause outbreaks of food poisoning or epidemic, therefore is essential to develop and adapt methods useful in epidemiological investigations. The aim of the study was to assess the genetic diversity of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples. METHODS: In this study it has been determined the genetic diversity of the pilot group of epidemiologically non- related 39 C. perfringens strains using RAPD (Random Amplified Polymorphic DNA), rep-PCR (Repetitive DNA Element Based PCR) and PFGE (Pulsed Field Gel Electrophoresis) methods. RESULTS AND CONCLUSIONS: It has been obtained comparable results of differentiation of C. perfringens strains isolated from patients with symptoms of food poisoning and food samples using RAOD and rep-PCR methods. It has been also confirmed a wide genetic diversity among C. perfringens strains isolated from different sources epidemiologically non-related using PFGE.
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