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  • Title: Detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure.
    Author: Andino RH, Torres HN, Polacino PS, Schudel A, Palma EL.
    Journal: Am J Vet Res; 1987 Jun; 48(6):984-7. PubMed ID: 3037953.
    Abstract:
    A sensitive and specific procedure for the detection of Argentine isolates of bovine herpesvirus-1 was developed. The procedure was based on a dot-blot, nucleic acid hybridization, using 32P, nick-translated, plasmidic probes. The probes contained cloned Bam H1 restriction fragments in the left half of the viral genome. The detection limit of the procedure was 10 pg of viral DNA.
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