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  • Title: Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA.
    Author: Ouattara M, Whaley MJ, Jenkins LT, Schwartz SB, Traoré RO, Diarra S, Collard JM, Sacchi CT, Wang X.
    Journal: Diagn Microbiol Infect Dis; 2019 Mar; 93(3):188-190. PubMed ID: 30413354.
    Abstract:
    This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput.
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