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  • Title: Whole-Genome Bisulfite Sequencing for the Methylation Analysis of Insect Genomes.
    Author: Gatzmann F, Lyko F.
    Journal: Methods Mol Biol; 2019; 1858():141-156. PubMed ID: 30414116.
    Abstract:
    DNA methylation is a conserved epigenetic modification of animal genomes, but genome methylation patterns appear surprisingly diverse in insects. Whole-genome bisulfite sequencing (WGBS) represents a sensitive and robust method for the characterization of genome-wide methylation patterns at single-base resolution. Here, we describe a step-by-step protocol for the generation and analysis of WGBS datasets using standard Illumina sequencing platforms. In comparison to whole-genome sequencing, WGBS has additional caveats that require particular attention and are highlighted in this chapter.
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