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  • Title: Isolation of Proteins from Polyacrylamide Gels.
    Author: Koristka S, Arndt C, Bergmann R, Bachmann M.
    Journal: Methods Mol Biol; 2019; 1855():461-465. PubMed ID: 30426439.
    Abstract:
    Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
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