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  • Title: Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis.
    Author: Thomas R, Kurien BT.
    Journal: Methods Mol Biol; 2019; 1855():491-494. PubMed ID: 30426443.
    Abstract:
    We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 min using heated (44 °C) Laemmli running buffer and high voltage.
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