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  • Title: Artifacts and Common Errors in Protein Gel Electrophoresis.
    Author: Kurien BT, Scofield RH.
    Journal: Methods Mol Biol; 2019; 1855():511-518. PubMed ID: 30426446.
    Abstract:
    In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating the cross-linking factor of a gel, polymerization temperature and time for a polyacrylamide gel, inducing aggregates in samples for electrophoresis, titrating the running buffer in electrophoresis, proper sample preparation, amount of protein to be loaded on a gel, sample buffer-to-protein ratios, incompletely removing phosphate-buffered saline from cells prior to cell lysis, and over-focusing of IPG strip in two-dimensional gel electrophoresis. In addition, subtle artifacts can have significant deleterious effects on carefully planned and executed experiments. Proteases that act at room temperature upon proteins in the sample buffer prior to heating, cleavage of the Asp-Pro bond upon prolonged heating of proteins at high temperatures, contamination of sample or sample buffer with keratin, leaching of chemicals from disposable plastic ware, and contamination of urea with ammonium cyanate are some of the common reasons for artifacts in gel electrophoresis. Taking proper heed to all these factors can greatly help generate good experimental results.
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