These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of phospholipase on cation-induced transmembrane transport of DNA in Escherichia coli].
    Author: Sabel'nikov AG, Mishina IA, Vasilenko IA, Il'iashenko BN.
    Journal: Mol Gen Mikrobiol Virusol; 1988 Mar; (3):43-8. PubMed ID: 3043211.
    Abstract:
    Incubation of bacterial cells in 0.1 M CaCl2 at 0 degrees C considerably increases the amount of phospholipids susceptible to action of a specific enzyme of phospholipid metabolism phospholipase C (hydrolysis to diacylglycerides). In process of incubation in CaCl2 solutions at 0 degrees C the expressed activity of an endogenous enzyme phospholipase A has been registered in cellular samples. Binding of the enzyme by the cells under conditions unfavourable for phospholipids hydrolysis (0 degrees C) suppresses strongly and reversibly cellular ability to DNA transformation without affecting cellular survival. As calculated, the enzyme molecules cover about 10% of cellular surface while inhibiting 90% of transmembrane transfer. The obtained data are considered to be a solid argument supporting the important role of the membrane phospholipids in the mechanism of cation-induced DNA transfer into the cell.
    [Abstract] [Full Text] [Related] [New Search]