These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Simple, Fast And Highly Sensitive Detection Of Gram-Negative Bacteria By A Novel Electrical Biosensor.
    Author: Wu J, Cheng C, Yuan Q, Oueslati R, Zhang J, Chen J, Almeida R.
    Journal: Annu Int Conf IEEE Eng Med Biol Soc; 2018 Jul; 2018():1279-1282. PubMed ID: 30440624.
    Abstract:
    This work presents a rapid, low-cost, highly sensitive and specific capacitive sensor for detection of Gram negative bacteria in a field setting. Recognition of Gramnegative bacteria is based on specific detection of lipopolysaccharides (LPS) by LPS-specific aptamer probe immobilized on electrode sensors. An inhomogeneous AC electric field is applied on sensor electrodes and induces positive dielectrophoresis that attracts LPS particles to the sensor electrodes for rapid detection. The same AC signal is also used to detect the binding reactions occurred on the sensor surface. The AC signal was optimized, and the binding between LPS and the specific aptamer was demonstrated. The detection limit reaches as low as 4.9 fg/mL for free LPS molecules and 53 #/mL of bacteria within a 30s' response time, meeting the needs of onsite bacteria detection.
    [Abstract] [Full Text] [Related] [New Search]