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  • Title: An improved procedure for shipboard enumeration of faecal indicator bacteria in marine sediments from sewage sludge disposal areas.
    Author: West PA.
    Journal: J Appl Bacteriol; 1988 Apr; 64(4):345-53. PubMed ID: 3049501.
    Abstract:
    An improved membrane filtration procedure for use on board ship to enumerate Escherichia coli and Group D faecal streptococci in marine sediments is described. Ultrasonication extraction combined with resuscitation of sublethally-injured cells yielded significantly higher counts of E. coli than sediments shaken by hand. Counts of E. coli were also higher on mFC agar (without rosalic acid) after a period of resuscitation on tryptone-soy agar supplemented with 0.1% yeast extract than on a 4% Teepol-lactose medium. Ultrasonication of sediments made no significant difference to counts of Group D faecal streptococci on KF-streptococcus agar. These improved isolation procedures allowed better discrimination of the area affected by sewage sludge at a disposal site off the northeast coast of England.
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