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  • Title: Sensitive aptamer-based fluorescene assay for ochratoxin A based on RNase H signal amplification.
    Author: Wu K, Ma C, Zhao H, Chen M, Deng Z.
    Journal: Food Chem; 2019 Mar 30; 277():273-278. PubMed ID: 30502145.
    Abstract:
    A new assay for the selective and sensitive aptamer-based fluorescent biosensor for ochratoxin A (OTA) detection is proposed. It consists of a ribonuclease H (RNase H)-assisted cycle response that leads to a significant amplification of the signal, which allow for a low limit of detection (LOD) of 0.08 ng/mL. By using this technique, a high selectivity for OTA against ochratoxin B and aflatoxin B1 (AFB1) was noticed. The quantitative determination in real samples has been verified using red wine samples spiked with a series of OTA concentrations (0.4, 4, and 12 ng/mL), and the recoveries ranged from 96.1 to 107.5%. This aptasensor has great practical applications in food industry and moreover, can be extended for the detection of other toxins by replacing the sequence of the recognition aptamer.
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