These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A naphthalene-based fluorescent probe for ratiometric imaging of lysosomal hydrogen sulfide in living cells.
    Author: Gao C, Liu X, Chen W, Wang F, Jiang JH.
    Journal: Methods Appl Fluoresc; 2018 Dec 17; 7(1):014002. PubMed ID: 30523933.
    Abstract:
    Hydrogen sulfide is an important gasotransmitter that exhibits various functions in physiological processes. We present a ratiometric fluorescence probe (SN-N3) for H2S by functionalizing naphthalene imide with 4-(azidomethyl)benzene as a H2S recognition moiety and morpholine moiety as a lysosomal targeting unit. After reaction with H2S, the azido moiety is reduced to amine group, and the probe releases the self-immolative linker and regenerates the fluorophore with internal charge transfer effect. SN-N3 is responsive to H2S in a ratiometric mode, exhibiting excellent sensitivity and high selectivity. The probe is demonstrated to be localized in lysosomes with high specificity. More importantly, SN-N3 is successfully demonstrated to image lysosomal H2S in a ratiometric manner. Our design provides a novel tool to image H2S in living cells that would hold great potential in exploring various H2S-related physiological and pathological cellular processes.
    [Abstract] [Full Text] [Related] [New Search]