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  • Title: Routine use of MALDI-TOF MS for anaerobic bacterial identification in clinical microbiology.
    Author: Shannon S, Kronemann D, Patel R, Schuetz AN.
    Journal: Anaerobe; 2018 Dec; 54():191-196. PubMed ID: 30541686.
    Abstract:
    In 2013, we adopted MALDI-TOF MS using the Bruker Biotyper system for identification of anaerobic bacteria into our routine clinical practice. Here, we describe our experience with the use of MALDI-TOF MS for anaerobic bacterial identification, highlighting its value in replacing the more costly and time-consuming 16S ribosomal RNA gene PCR plus sequencing-based approach as the primary method of anaerobic bacterial identification. We also describe our more recent experience with the use of early/rapid MALDI-TOF MS for identification of anaerobic bacteria performed on short incubation (4-6 h) plated aerobic media from anaerobic blood culture bottles positive for Gram-negative bacilli.
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