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  • Title: Enhancement of luminol-based immunodot and Western blotting assays by iodophenol.
    Author: Leong MM, Fox GR.
    Journal: Anal Biochem; 1988 Jul; 172(1):145-50. PubMed ID: 3056094.
    Abstract:
    The application of 4-iodophenol as a signal enhancer for luminol-based immunodot and Western blotting assays was investigated. Immunodetection of biotin-bovine serum albumin (BSA) using a rat anti-biotin antibody for immunodot binding and goat anti-biotin peroxidase conjugate for Western blotting assays was employed to demonstrate the signal enhancement attainable with 4-iodophenol. The addition of 4-iodophenol at a final working concentration of 52.2 microM to a light-emitting substrate, luminol, resulted in 3-fold and 10-fold signal enhancements for Western blotting and immunodot binding detection of biotin-BSA, respectively.
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