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  • Title: Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression.
    Author: Yamaguchi R, Matsuo K, Yamazaki A, Nagai S, Terasaka K, Yamada T.
    Journal: FEBS Lett; 1988 Nov 21; 240(1-2):115-7. PubMed ID: 3056744.
    Abstract:
    Using a single-probe method, we have cloned the gene for an immunogenic MPB57 protein of Mycobacterium bovis BCG. The nucleotide sequence includes an ORF of 300 base pairs encoding a protein of 99 amino acids with an Mr of 10,818. This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57.
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