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  • Title: Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli.
    Author: Aoyama S, Yoshida N, Inouye S.
    Journal: FEBS Lett; 1988 Dec 19; 242(1):36-40. PubMed ID: 3060375.
    Abstract:
    The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.
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