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  • Title: A label-free and highly sensitive DNA biosensor based on the core-shell structured CeO2-NR@Ppy nanocomposite for Salmonella detection.
    Author: Nguyet NT, Yen LTH, Doan VY, Hoang NL, Van Thu V, Lan H, Trung T, Pham VH, Tam PD.
    Journal: Mater Sci Eng C Mater Biol Appl; 2019 Mar; 96():790-797. PubMed ID: 30606592.
    Abstract:
    A core-shell cerium oxide nanorod@polypyrrole (CeO2-NR@Ppy) nanocomposite-based electrochemical DNA biosensor was studied for Salmonella detection. The core-shell CeO2-NR@Ppy nanocomposite was prepared by in situ chemical oxidative polymerization of pyrrole monomer on CeO2-NRs, which provided a suitable platform for electrochemical DNA biosensor fabrication. The immobilization of ss-DNA sequences onto nanocomposite-coated microelectrode was performed via covalent attachment method. DNA biosensor electrochemical responses were studied by cyclic voltammetry and electrochemical impedance spectroscopy with [Fe (CN)6]3-/4- as redox probe. Under optimal conditions, DNA biosensor response showed good linearity in the range of 0.01-0.4 nM with sensitivity of 593.7 Ω·nM-1·cm-2. The low limit of detection and limit of quantification for the DNA biosensor were 0.084 and 0.28 nM, respectively. The proposed DNA biosensor also showed good results when used in detecting actual Salmonella samples.
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