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  • Title: An improved assay method for amylase activity using an amylodextrin fraction as substrate.
    Author: Kikumoto S.
    Journal: Carbohydr Res; 1978 Mar; 61():369-75. PubMed ID: 306285.
    Abstract:
    A rapid and simple method for determination of amylase activity, by using a fraction of amylo-oligosaccharide (amylodextrin, 1) as substrate, is described. The sample solution is incubated with a solution of 1 and the substrate consumed is estimated by measuring the difference in absorbance at 460 nm. The Km value of 1 is about half that of starch. The homogeneity of 1 in its chemical structure and molecular weight facilitated the specification of amylase units according to the international definition. This procedure was applied for assay of human-serum amylase with excellent reproducibility. This method did not require a large dilution factor, as the standard curve showed a linear relationship over a wide range of amylase concentrations.
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