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  • Title: Cloning and sequencing the HinfI restriction and modification genes.
    Author: Chandrasegaran S, Lunnen KD, Smith HO, Wilson GG.
    Journal: Gene; 1988 Oct 30; 70(2):387-92. PubMed ID: 3063606.
    Abstract:
    The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).
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