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  • Title: Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.
    Author: Fedor MJ, Lue NF, Kornberg RD.
    Journal: J Mol Biol; 1988 Nov 05; 204(1):109-27. PubMed ID: 3063825.
    Abstract:
    Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific.
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