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  • Title: Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine.
    Author: Attar AM, Richardson MB, Speciale G, Majumdar S, Dyer RP, Sanders EC, Penner RM, Weiss GA.
    Journal: ACS Appl Mater Interfaces; 2019 Feb 06; 11(5):4757-4765. PubMed ID: 30668098.
    Abstract:
    A polymer-based electrode capable of specific detection of human serum albumin, and its glycated derivatives, is described. The sensor is constructed from a glass microscope slide coated with a synthesized, polythiophene film bearing a protected, iminodiacetic acid motif. The electrode surface is then further elaborated to a functional biosensor through deprotection of the iminodiacetic acid, followed by metal-affinity immobilization of a specific and high-affinity, albumin ligand. Albumin was then quantified in buffer and synthetic urine via electrochemical impedance spectroscopy. Glycated albumin was next bound to a boronic acid-modified, single-cysteine dihydrofolate reductase variant to quantify glycation ratios by square-wave voltammetry. The platform offers high sensitivity, specificity, and reproducibility in an inexpensive arrangement. The detection limits exceed the requirements for intermediate-term glycemic control monitoring in diabetes patients at 5 and 1 nM for albumin and its glycated forms, respectively.
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