These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination.
    Author: Stirling CJ, Stewart G, Sherratt DJ.
    Journal: Mol Gen Genet; 1988 Sep; 214(1):80-4. PubMed ID: 3067082.
    Abstract:
    The heritable stability of the multicopy plasmid ColE1 and its natural relatives, requires the presence in the plasmid of a site (cer in ColE1) that acts as a substrate for site-specific recombination, thereby maintaining plasmids in the monomeric state. Multimerization, promoted by homologous recombination, leads to plasmid loss. Here we show that the Escherichia coli chromosome encodes at least two unlinked functions that act on cer and its analogous sites, to promote stabilizing site-specific recombination. One of these functions is encoded by a gene residing on a cosmid that also contains the argI and pyrB genes, mapping it to the 96-97 min region of the E. coli map.
    [Abstract] [Full Text] [Related] [New Search]