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  • Title: Quantitative determination of cross-linkage of bacteriophage DNA and protein by ionizing radiation.
    Author: Hawkins RB.
    Journal: Int J Radiat Biol Relat Stud Phys Chem Med; 1978 May; 33(5):425-41. PubMed ID: 306977.
    Abstract:
    Coliphage T7 was dissolved in tryptone broth and exposted to 60C gamma-radiation. Cross-linkage of DNA and protein of the virion was assayed using phenol-water countercurrent distribution. The results are interpreted in terms of a statistical model of cross-linkage and double-strand breaks. It was found that protein--DNA cross-links accumulate linearly with dose at a rate of o.74 X 10(-11) cross-links per rad per nucleotide pair, which is of the order of 5 per cent of the formation rate of double-strand breaks.
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