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  • Title: Label-free detection of exosomes using a surface plasmon resonance biosensor.
    Author: Sina AA, Vaidyanathan R, Wuethrich A, Carrascosa LG, Trau M.
    Journal: Anal Bioanal Chem; 2019 Mar; 411(7):1311-1318. PubMed ID: 30719562.
    Abstract:
    The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs, and proteins) to remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label-free detection of clinically relevant exosomes using a surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell-derived exosomes particularly from complex biological samples (e.g. exosome spiked in serum). This approach exhibits high sensitivity by detecting as low as 8280 exosomes/μL which may potentially be suitable for clinical analysis. We believe that this label-free and real-time method along with the high specificity and sensitivity may potentially be useful for clinical settings.
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