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  • Title: Rat sperm protamine. Isolation and sequence analysis.
    Author: Ammer H, Henschen A.
    Journal: Biol Chem Hoppe Seyler; 1988 Dec; 369(12):1301-6. PubMed ID: 3072011.
    Abstract:
    Rat protamine was extracted from S-(pyridylethylated) epididymal sperm cell nuclei with dilute hydrochloric acid. The final purification was achieved by reversed-phase high-performance liquid chromatography. The primary structure was determined by N-terminal sequencing of the total S-(pyridylethylated) protein, and of endoproteinase Lys-C- and thermolysin-derived fragments. Rat protamine consists of 50 amino-acid residues. It is a typical type 1 protamine and differs in two and ten positions from the corresponding mouse and rabbit protamine, respectively. Only 26 positions are invariant in all type 1 mammalian protamines.
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