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  • Title: [Continuous production of anti-hepatitis B surface antigen monoclonal antibody in hollow fiber perfusion bioreactor].
    Author: Meng MH, Chen GC, Hsieh JH, Yueh Y, Chen SC, Chang TH, Tsao D.
    Journal: Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi; 1988 Aug; 21(3):125-40. PubMed ID: 3072154.
    Abstract:
    Hybridoma cell line H440Ac was cultivated continuously in hollow fiber perfusion bioreactor for fifty days to produce anti-hepatitis B surface antigen (anti-HBsAg) monoclonal antibody. A total of 90 mg anti-HBsAg antibody was produced in the hollow fiber bioreactor with 1000 cm2 membrane surface area. The collected production medium has an average antibody concentration of 0.45 mg/ml with a high concentration of 2.3 mg/ml. This compares with 0.016 and 1.1 mg/ml antibody concentration as collected from spinner flask and mouse ascites. The purity of the produced antibody is 0.085, 0.0028 and 0.026 mg antibody per mg of total harvested protein in the culture media produced from the hollow fiber bioreactor, spinner flasks and mouse ascites, respectively.
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