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  • Title: Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China.
    Author: Liu C, Li M, Redda ET, Mei J, Zhang J, Elena SF, Wu B, Jiang X.
    Journal: Arch Virol; 2019 Apr; 164(4):1213-1216. PubMed ID: 30746561.
    Abstract:
    A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus "Trichoderma harzianum bipartite mycovirus 1" (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.
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