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  • Title: Glucose-stimulated phosphorylation of yeast isocitrate lyase in vivo.
    Author: López-Boado YS, Herrero P, Fernández T, Fernández R, Moreno F.
    Journal: J Gen Microbiol; 1988 Sep; 134(9):2499-505. PubMed ID: 3076186.
    Abstract:
    Incorporation of 32P into Saccharomyces cerevisiae isocitrate lyase was observed after addition of glucose to a culture incubated with [32P]orthophosphoric acid. A band of 32P-labelled protein was coincident with the enzyme band when immunoprecipitates were subjected to SDS-PAGE and autoradiography. No label was found in the band corresponding to the isocitrate lyase when immunoprecipitation was done with a control pre-immune serum or in the presence of excess pure unlabelled enzyme. The incorporation of phosphate was associated with a decrease in enzyme activity. Phosphorylated isocitrate lyase was not proteolytically degraded when cells were cultured in mineral medium. The loss of protein antigenicity only took place when the yeast was grown in a complex medium containing glucose.
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