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  • Title: Black phosphorus nanosheets based sensitive protease detection and inhibitor screening.
    Author: Hu Z, Li Y, Hussain E, Huang X, Zhang Y, Niu N, Shahzad SA, Yu C.
    Journal: Talanta; 2019 May 15; 197():270-276. PubMed ID: 30771934.
    Abstract:
    Proteases, as one of the most significant kind of digestive enzymes, are closely related to a variety of physiological processes and diseases. Herein, a black phosphorus nanosheets (BPs) based sensitive fluorometric method for protease detection and inhibitor screening is proposed. The aqueous solution of perylene probe (probe 1) displays a strong fluorescence. BPs, as novel discovered two-dimensional (2D) materials, can adsorb probe 1 through electrostatic interactions, which causes fluorescence quenching of probe 1. Histone can control the interactions between the perylene probe and BPs, which can be further regulated by the introduction of a protease. Thus, the protease activity can be monitored by detecting the fluorescence intensity changes of probe 1. The method is label free, sensitive and selective. As low as 1 ng/mL trypsin can be easily detected.
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