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  • Title: Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.
    Author: LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R.
    Journal: J Bacteriol; 1986 Jan; 165(1):204-14. PubMed ID: 3079748.
    Abstract:
    Integrable plasmids pGR102 and pWR103 containing ribosomal sequences from within the transcriptional units for 16S and 23S were used to transform Bacillus subtilis. To date, these plasmids integrated into 7 of 10 known rrn operons. Two such events occurred at unassigned operons, revealing a close linkage of the CAT gene of the plasmid to pha-1 situated between dal-1 and purB33 for rrnE and to thiA78 situated between glyB133 and re-12 for rrnD. All seven integration events that led to the loss of unique ribosomal BclI fragments can now be assigned to known rrn operons.
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