These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.
    Author: Mumby SM, Kahn RA, Manning DR, Gilman AG.
    Journal: Proc Natl Acad Sci U S A; 1986 Jan; 83(2):265-9. PubMed ID: 3079909.
    Abstract:
    Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all alpha or all beta subunits from Gs, Gi, Go, and transducin; others recognized only Gs alpha or Go alpha. Such cross-reaction or complete specificity for a given alpha subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.
    [Abstract] [Full Text] [Related] [New Search]