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  • Title: Acetylcholine release from guinea pig caudate slices evoked by phorbol ester and calcium.
    Author: Tanaka C, Fujiwara H, Fujii Y.
    Journal: FEBS Lett; 1986 Jan 20; 195(1-2):129-34. PubMed ID: 3080330.
    Abstract:
    In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate) produced an increase in the Ca2+-dependent, depolarization-evoked release of acetylcholine (ACh), with no significant effect on the evoked Ca2+ influx. The effect of TPA was antagonized by polymyxin B and H-7, both inhibitors of protein kinase C. The combination of TPA and calcium ionophore (A23187) provoked the release of ACh to a level equal to the maximal response induced by depolarizing stimuli. TPA alone had no significant effect on the release of ACh. These results suggest that protein kinase C probably plays a role in transmembrane signal transduction involved in the release of neurotransmitter from nerve terminals in the brain.
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