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  • Title: Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection.
    Author: Jiang S, Zhang Y, Yang Y, Huang Y, Ma G, Luo Y, Huang P, Lin J.
    Journal: ACS Appl Mater Interfaces; 2019 Mar 20; 11(11):10554-10558. PubMed ID: 30807088.
    Abstract:
    The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC-GOx/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC-GOx/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC-GOx/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.
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