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  • Title: Evaluating formulation and storage of Arthrobacter aurescens strain TC1 as a bioremediation tool for terbuthylazine contaminated soils: Efficacy on abatement of aquatic ecotoxicity.
    Author: Viegas CA, Silva VP, Varela VM, Correia V, Ribeiro R, Moreira-Santos M.
    Journal: Sci Total Environ; 2019 Jun 10; 668():714-722. PubMed ID: 30856579.
    Abstract:
    Recently, a bioremediation tool consisting of freshly grown cells of the Arthrobacter aurescens strain TC1 proved successful in the cleanup of terbuthylazine (TBA) contaminated soil, with potential to prevent TBA dispersal via the soil-water pathway. The present work aimed at examining the feasibility of preparing and formulating bioaugmentation bacterial cells in advance which retain adequate viability and herbicide-degrading activity during storage and transport to contaminated sites. Three different types of formulation of A. aurescens TC1 cells were performed, namely cell paste, lyophilized, and adsorbed onto the mineral carrier vermiculite. Vermiculite-based cell material and cell paste offered survival rates significantly higher (≥78% of cells recovered viable, when compared to the initial numbers) than lyophilized cells (≥65%) after storage at 4 °C for up to a maximum period of 30 days. Inocula prepared from the three types of formulated and stored cells supported adequate levels of herbicide-biodegradation activity, ultimately allowing an almost complete removal of TBA from either liquid buffer or soil. In soil microcosms, the efficacy of bioremediation of TBA-contaminated soil in terms of decontamination (%) based on the ecotoxicity assessment of soil eluates towards a freshwater microalga was found to differ within formulations in the order: lyophilized cells (~100% efficacy at the end of one or two weeks of bioremediation treatment with an initial inoculum of ~2.5 × 107 viable cells/g of soil; similar to fresh cells) > vermiculite-adsorbed cells (81% with ~2.4 × 108 cells/g) > cell paste (68% with ~2.4 × 108 cells/g). The limitations and advantages of each type of bacterial formulation, together with the prediction of their applicability in the field are discussed.
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