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  • Title: Lipopolysaccharide changes and cytoplasmic polyphosphate granule accumulation in Pseudomonas aeruginosa during growth on hexadecane.
    Author: Miguez CB, Beveridge TJ, Ingram JM.
    Journal: Can J Microbiol; 1986 Mar; 32(3):248-53. PubMed ID: 3085910.
    Abstract:
    Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did not adhere to hexadecane droplets suggesting the absence of a bioemulsifier. When compared with glucose-grown cells, hexadecane-grown cells produced 75% less lipopolysaccharide (on a total protein basis); this lipopolysaccharide contained 30-40% less carbohydrate, yet 50-75% more 2-keto-3-deoxyoctonate. These chemical changes made the cell surface appear more hydrophobic when tested in a biphasic hydrophobicity index system. Electron microscopy of thin sections and freeze etchings revealed hexadecane-grown cells contained granules which were judged to be polyphosphate by energy dispersive X-ray analysis. There was no apparent major morphological envelope alteration within the two cell types.
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