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  • Title: Molecular detection of Histoplasma capsulatum indoors: A public health approach.
    Author: Amorim-Conselheiro J, Orico LD, Galvão-Dias MA, Grigorio IM, Neto HM, Da Rosa AR, Oliveira DC, Taborda CP, Reis-Menezes AA.
    Journal: Rev Iberoam Micol; 2019; 36(2):79-82. PubMed ID: 30862390.
    Abstract:
    BACKGROUND: Histoplasmosis, caused by the dimorphic fungus Histoplasma capsulatum, represents an important public health problem, especially in urban environments where bats and humans cohabit indoors. AIMS: To detect the presence of H. capsulatum indoors, using samples of bat droppings collected in roost sites inside houses. METHODS: A Real-Time TaqMan PCR assay targeting the ITS1 region of the ribosomal DNA of H. capsulatum was carried out. RESULTS: Fifty-nine sampling points in the municipality of São Paulo were inspected, all of them located at inhabited places. H. capsulatum was isolated from nine samples. CONCLUSIONS: The rapid identification and monitoring of sites where the fungus is present may contribute to make a more reliable database of H. capsulatum distribution.
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