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Title: Evaluation of Plating Media for Recovery of Heated Clostridium perfringens Spores. Author: Labbe RG, Norris KE. Journal: J Food Prot; 1982 Jun; 45(8):686-688. PubMed ID: 30866204. Abstract: Four selective and eight non-selective plating media were evaluated for their ability to enumerate six strains of heat-activated and heat-injured spores of Clostridium perfringens . Trypticase-sulfite-neomycin (TSN) agar and sulfite-polymyxin-sulfadiazine (SPS) agar gave higher counts of heat-activated spores than non-selective media. In the case of heat-injured spores, wide variation in recovery was obtained depending on strain and medium. Higher counts of heat-injured spores were obtained by incubating plates at 37°C than at 45°C, although, except for one strain, no significant difference between the two temperatures was observed using heat-activated spores.[Abstract] [Full Text] [Related] [New Search]