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  • Title: Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor.
    Author: Kampeera J, Pasakon P, Karuwan C, Arunrut N, Sappat A, Sirithammajak S, Dechokiattawan N, Sumranwanich T, Chaivisuthangkura P, Ounjai P, Chankhamhaengdecha S, Wisitsoraat A, Tuantranont A, Kiatpathomchai W.
    Journal: Biosens Bioelectron; 2019 May 01; 132():271-278. PubMed ID: 30878727.
    Abstract:
    Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.
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