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  • Title: A clone coding for Schizophyllum commune beta-glucosidase: homology with a yeast beta-glucosidase.
    Author: Moranelli F, Barbier JR, Dove MJ, MacKay RM, Seligy VL, Yaguchi M, Willick GE.
    Journal: Biochem Int; 1986 Jun; 12(6):905-12. PubMed ID: 3091028.
    Abstract:
    Three identical clones coding for a partial sequence of the Schizophyllum commune beta-glucosidase were isolated from a cDNA library in lambda gt11, using polyclonal antibody to the enzyme. The identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-C digest with the sequence inferred from the cDNA sequence. A comparison of the sequence with that reported for a beta-glucosidase from Candida pelliculosa revealed a region in the latter with 43% identity in amino acid sequence. There was also a similarity in the S. commune beta-glucosidase to an active site sequence proposed for a S. commune endoglucanase, suggesting the possibility of a common catalytic mechanism for these two glucolytic enzymes.
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