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  • Title: A region of the immunoglobulin-mu heavy chain necessary for forming pentameric IgM.
    Author: Baker MD, Wu GE, Toone WM, Murialdo H, Davis AC, Shulman MJ.
    Journal: J Immunol; 1986 Sep 01; 137(5):1724-8. PubMed ID: 3091689.
    Abstract:
    In order to define the molecular requirements for IgM pentamer formation, we have isolated several mutant hybridomas which produce predominantly monomeric IgM. For one such mutant, 102, we synthesized a cDNA clone of its mu-mRNA, and found an in-frame 39-bp deletion, which thus encodes a mu-chain lacking amino acids 550-562, a region spanning the fourth constant domain and the tail of the mu-chain. To prove that this deletion is sufficient to block pentamer formation, we used site-directed mutagenesis to construct a mu-gene lacking these 39 bp, and have shown that the expression of this altered mu-gene results in the production of monomeric IgM.
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